Rfx2 (NM_009056) Mouse Recombinant Protein

Product Specifications

Background

Transcription factor that acts as a key regulator of spermatogenesis (PubMed:26248850, PubMed:26162102, PubMed:26853561) . Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (PubMed:26162102, PubMed:26853561) . Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC (0-3N) RGYAAC-3' present on promoters (PubMed:15229132, PubMed:26162102) . Probably activates transcription of the testis-specific histone gene HIST1H1T (PubMed:15229132) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse regulatory factor X, 2 (influences HLA class II expression) (Rfx2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

5430432H19Rik

Gene ID

19725

UniProt

P48379

Accession Number mRNA

NM_009056

Chromosomal Location

17 29.5 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

77 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.