Pcif1 (NM_146129) Mouse Recombinant Protein
Product Specifications
Background
Cap-specific adenosine methyltransferase that catalyzes formation of N (6),2'-O-dimethyladenosine cap (m6A (m) ) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A (m) co-transcriptionally. N6-methylation of m6A (m) promotes the translation of capped mRNAs.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse PDX1 C-terminal inhibiting factor 1 (Pcif1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2310022K11Rik; C20orf67; F730014I05Rik
Gene ID
228866
UniProt
P59114, Q542C3
Accession Number mRNA
NM_146129
Chromosomal Location
2 H3
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
80.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Pcif1
Species
Mouse
Protein ID
NP_666241
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/228866
Uniprot URL
https://www.uniprot.org/uniprot/P59114
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_146129
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_666241
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