Pcif1 (NM_146129) Mouse Recombinant Protein

Product Specifications

Background

Cap-specific adenosine methyltransferase that catalyzes formation of N (6),2'-O-dimethyladenosine cap (m6A (m) ) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A (m) co-transcriptionally. N6-methylation of m6A (m) promotes the translation of capped mRNAs.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse PDX1 C-terminal inhibiting factor 1 (Pcif1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2310022K11Rik; C20orf67; F730014I05Rik

Gene ID

228866

UniProt

P59114, Q542C3

Accession Number mRNA

NM_146129

Chromosomal Location

2 H3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

80.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.