Ncl (NM_010880) Mouse Recombinant Protein
Product Specifications
Background
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity) .[UniProtKB/Swiss-Prot Function]
Synonyms
B530004O11Rik; C23; D0Nds28; D1Nds28; Nucl
Gene ID
17975
UniProt
P09405
Accession Number mRNA
NM_010880
Chromosomal Location
1 43.94 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
76.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Ncl
Species
Mouse
Protein ID
NP_035010
Overview
Purified recombinant protein of Mouse nucleolin (Ncl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/17975
Uniprot URL
https://www.uniprot.org/uniprot/P09405
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_010880
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_035010
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