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Spire2 (NM_172287) Mouse Recombinant Protein

Product Specifications

Background

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703, PubMed:21983562) . Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562) . Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis (PubMed:21620703) . Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703) . Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse spire type actin nucleation factor 2 (Spire2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

BC026502; Spir-2; Spir2

Gene ID

234857

UniProt

Q8K1S6

Accession Number mRNA

NM_172287

Chromosomal Location

8 E1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

80.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Spire2

Species

Mouse

Protein ID

NP_758491

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/234857

Uniprot URL

https://www.uniprot.org/uniprot/Q8K1S6

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_172287

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_758491

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