Mcm3 (BC031700) Mouse Recombinant Protein
Product Specifications
Background
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse minichromosome maintenance deficient 3 (S, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
P1, p1.m
Gene ID
17215
UniProt
P25206
Accession Number mRNA
BC031700
Chromosomal Location
1 A4
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
91.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Mcm3
Species
Mouse
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/17215
Uniprot URL
https://www.uniprot.org/uniprot/P25206
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/BC031700
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