Usp4 (BC011341) Mouse Recombinant Protein

Product Specifications

Background

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

F730026I20Rik; mKIAA4155; Unp

Gene ID

22258

UniProt

P35123

Accession Number mRNA

BC011341

Chromosomal Location

9 59.25 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

126.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.