Arhgef1 (NM_001130152) Mouse Recombinant Protein
Product Specifications
Background
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
Lbcl2; Lsc
Gene ID
16801
UniProt
Q61210
Accession Number mRNA
NM_001130152
Chromosomal Location
7 A3
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
102.8 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Arhgef1
Species
Mouse
Protein ID
NP_001123624
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/16801
Uniprot URL
https://www.uniprot.org/uniprot/Q61210
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001130152
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001123624
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
