Nos3 (BC052636) Mouse Recombinant Protein

Product Specifications

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

ENOS, ecNOS

Gene ID

18127

UniProt

P70313

Accession Number mRNA

BC052636

Chromosomal Location

5 11.32 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

146 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.