Sf3b3 (NM_133953) Mouse Recombinant Protein

Product Specifications

Background

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse splicing factor 3b, subunit 3 (Sf3b3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1810061H24Rik; 5730409A01Rik; AA409318; D8Ertd633e; mKIAA0017; RSE1; SAP130

Gene ID

101943

UniProt

Q921M3, B2RSV4

Accession Number mRNA

NM_133953

Chromosomal Location

8 57.73 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

136 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.