Dhx29 (NM_172594) Mouse Recombinant Protein

Product Specifications

Background

ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 29 (Dhx29), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

3732415M03; AU043276; Ddxx; E130202M19Rik

Gene ID

218629

UniProt

Q6PGC1

Accession Number mRNA

NM_172594

Chromosomal Location

13 D2.2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

154.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.