Fbxo3 (NM_020593) Mouse Recombinant Protein

Product Specifications

Background

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) -type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse F-box protein 3 (Fbxo3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1200002G09Rik; 1700026K02Rik; AI046358; Fba

Gene ID

57443

UniProt

Q9DC63, Q9CVV6

Accession Number mRNA

NM_020593

Chromosomal Location

2 E2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

47.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.