Ppie (NM_019489) Mouse Recombinant Protein
Product Specifications
Background
Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly (A) -region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2010010D16Rik; Cyp33
Gene ID
56031
UniProt
Q9QZH3
Accession Number mRNA
NM_019489
Chromosomal Location
4 D2.2
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
33.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Ppie
Species
Mouse
Protein ID
NP_062362
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/56031
Uniprot URL
https://www.uniprot.org/uniprot/Q9QZH3
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_019489
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_062362
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