Ppie (NM_019489) Mouse Recombinant Protein

Product Specifications

Background

Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly (A) -region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2010010D16Rik; Cyp33

Gene ID

56031

UniProt

Q9QZH3

Accession Number mRNA

NM_019489

Chromosomal Location

4 D2.2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

33.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.