Klhl40 (NM_028202) Mouse Recombinant Protein

Product Specifications

Background

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development (PubMed:25940086) . The BCR (KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex (PubMed:25940086) . Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear (PubMed:24960163) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse kelch-like 40 (Klhl40), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2310024D23Rik; Kbtbd5

Gene ID

72330

UniProt

Q9D783, A0A0R4J166

Accession Number mRNA

NM_028202

Chromosomal Location

9 F4

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

70.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.