Polh (NM_030715) Mouse Recombinant Protein

DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10871396) . Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide (s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d (GpG) -cisplatin cross-link, inserts dCTP opposite the 3' guanine (By similarity) . Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10871396) . Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci (By similarity) .[UniProtKB/Swiss-Prot Function]

Purified recombinant protein of Mouse polymerase (DNA directed), eta (RAD 30 related) (Polh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.