Mocs3 (NM_001160330) Mouse Recombinant Protein
Product Specifications
Background
Plays a central role in 2-thiolation of mcm (5) S (2) U at tRNA wobble positions of cytosolic tRNA (Lys), tRNA (Glu) and tRNA (Gln) . Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.[UniProtKB/Swiss-Prot Function]
Synonyms
1700020H17Rik; Uba4
Gene ID
69372
UniProt
A2BDX3
Accession Number mRNA
NM_001160330
Chromosomal Location
2 H3
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
49.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Mocs3
Species
Mouse
Protein ID
NP_001153802
Overview
Purified recombinant protein of Mouse molybdenum cofactor synthesis 3 (Mocs3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/69372
Uniprot URL
https://www.uniprot.org/uniprot/A2BDX3
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001160330
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001153802
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