Pla2g2f (NM_012045) Mouse Recombinant Protein

Product Specifications

Background

May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases. Phospholipase A2, which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:10531313) . Hydrolyzes phosphatidylethanolamine more efficiently than phosphatidylcholine, with only a modest preference for arachidonic acid versus linoelic acid at the sn-2 position. Comparable activity toward 1-palmitoyl-2-oleoyl-phosphatidylserine vesicles to that toward 1-palmitoyl-2-oleoyl-phosphatidylglycerol (PubMed:11877435) . Prefers phosphatidylglycerol compared to phosphatidylcholine (PubMed:10531313) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse phospholipase A2, group IIF (Pla2g2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

GIIFsPLA2; sPLA2-IIF

Gene ID

26971

UniProt

Q9QZT4

Accession Number mRNA

NM_012045

Chromosomal Location

4 D3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

23.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.