Dock8 (NM_028785) Mouse Recombinant Protein
Product Specifications
Background
Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490) . During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (PubMed:22461490, PubMed:25713392) . Required for CD4 (+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151) . Is involved in NK cell cytotoxicity controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (By similarity) .[UniProtKB/Swiss-Prot Function]
Synonyms
1200017A24Rik; 5830472H07Rik; A130095G14Rik; AI461977
Gene ID
76088
UniProt
Q8C147
Accession Number mRNA
NM_028785
Chromosomal Location
19 B
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
239.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Dock8
Species
Mouse
Protein ID
NP_083061
Overview
Purified recombinant protein of Mouse dedicator of cytokinesis 8 (Dock8), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/76088
Uniprot URL
https://www.uniprot.org/uniprot/Q8C147
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_028785
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_083061
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