Ankrd16 (NM_177268) Mouse Recombinant Protein

Product Specifications

Background

Required to prevent the misactivation of serine (Ser) with tRNA (Ala) by promoting the hydrolysis of Ser-mischarged tRNA (Ala), thereby playing a role in translational fidelity (PubMed:29769718) . Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA (Ala) and precluding Ser misincorporation in nascent peptides (PubMed:29769718) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse ankyrin repeat domain 16 (Ankrd16), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2810455F06Rik; AI646698; D430029B21Rik

Gene ID

320816

UniProt

A2AS55

Accession Number mRNA

NM_177268

Chromosomal Location

2 A1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

39.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.