Ankrd16 (NM_177268) Mouse Recombinant Protein
Product Specifications
Background
Required to prevent the misactivation of serine (Ser) with tRNA (Ala) by promoting the hydrolysis of Ser-mischarged tRNA (Ala), thereby playing a role in translational fidelity (PubMed:29769718) . Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA (Ala) and precluding Ser misincorporation in nascent peptides (PubMed:29769718) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse ankyrin repeat domain 16 (Ankrd16), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2810455F06Rik; AI646698; D430029B21Rik
Gene ID
320816
UniProt
A2AS55
Accession Number mRNA
NM_177268
Chromosomal Location
2 A1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
39.8 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Ankrd16
Species
Mouse
Protein ID
NP_796242
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/320816
Uniprot URL
https://www.uniprot.org/uniprot/A2AS55
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_177268
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_796242
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