A1cf (NM_001081074) Mouse Recombinant Protein

Product Specifications

Background

Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse APOBEC1 complementation factor (A1cf), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1810073H04Rik; Acf; ACF64; ACF65; ASP; MCM; mer; MerCreMer; Tg (Myh6-cre/Esr1*) 1Jmk

Gene ID

69865

UniProt

Q5YD48

Accession Number mRNA

NM_001081074

Chromosomal Location

19 C1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

65.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.