A1cf (NM_001081074) Mouse Recombinant Protein
Product Specifications
Background
Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse APOBEC1 complementation factor (A1cf), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
1810073H04Rik; Acf; ACF64; ACF65; ASP; MCM; mer; MerCreMer; Tg (Myh6-cre/Esr1*) 1Jmk
Gene ID
69865
UniProt
Q5YD48
Accession Number mRNA
NM_001081074
Chromosomal Location
19 C1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
65.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
A1cf
Species
Mouse
Protein ID
NP_001074543
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/69865
Uniprot URL
https://www.uniprot.org/uniprot/Q5YD48
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001081074
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001074543
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