Pou3f2 (NM_008899) Mouse Recombinant Protein
Product Specifications
Background
Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019) . Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity) . The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220) . Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse POU domain, class 3, transcription factor 2 (Pou3f2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
9430075J19Rik; A230098E07Rik; Brn-2; Brn2; oct-7; OTF-7; Otf7
Gene ID
18992
UniProt
P31360
Accession Number mRNA
NM_008899
Chromosomal Location
4 9.73 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
47.6 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Pou3f2
Species
Mouse
Protein ID
NP_032925
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/18992
Uniprot URL
https://www.uniprot.org/uniprot/P31360
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_008899
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_032925
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