Phlpp1 (NM_133821) Mouse Recombinant Protein

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity) . Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity) . Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity) . Involved in the hippocampus-dependent long-term memory formation (PubMed:17382888) . Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (PubMed:20080691) . Involved in development and function of regulatory T-cells (PubMed:21498666) .[UniProtKB/Swiss-Prot Function]

Purified recombinant protein of Mouse PH domain and leucine rich repeat protein phosphatase 1 (Phlpp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.