Pappa (NM_021362) Mouse Recombinant Protein

Product Specifications

Background

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse pregnancy-associated plasma protein A (Pappa), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

8430414N03Rik; IGFBP-4ase; PAG1; PAPP-A

Gene ID

18491

UniProt

Q8R4K8

Accession Number mRNA

NM_021362

Chromosomal Location

4 34.14 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

181.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.