Thymidine Kinase 1 (TK1) (NM_003258) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

Overview

Recombinant protein of human thymidine kinase 1, soluble (TK1), expressed in human cells

Synonyms

TK2

Gene ID

7083

UniProt

P04183, A0A384MDV9

Accession Number mRNA

NM_003258

Chromosomal Location

17q25.3

Expression System

HEK293

Tag

C-DDK

Related Pathways

Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism

Field of Research

Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome, Stem cell - Pluripotency

Molecular Weight

27 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.