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RAD52 (NM_134424) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Synonyms

RP11-359B12.2

Gene ID

5893

UniProt

P43351, Q5DR82

Accession Number mRNA

NM_134424

Chromosomal Location

12p13.33

Expression System

HEK293

Tag

C-Myc/DDK

Related Pathways

Homologous recombination

Field of Research

Homologous recombination

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Function

Druggable Genome

Molecular Weight

46 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

RAD52

Species

Human

Protein ID

NP_602296

Overview

Purified recombinant protein of Human chromosome 15 open reading frame 23 (C15orf23), transcript variant 3, mutant (S24F), expressed in HEK293 cells, 20ug

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/5893

Uniprot URL

https://www.uniprot.org/uniprot/P43351

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_134424

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_602296

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