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Von Willebrand Factor (VWF) (NM_000552) Human Recombinant Protein

Product Specifications

Background

This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015]

Overview

Recombinant protein of human von Willebrand factor (VWF), residues 2200-2813aa, with C-terminal DDK tag, expressed in sf9 cells

Synonyms

F8VWF; VWD

Gene ID

7450

UniProt

P04275

Accession Number mRNA

NM_000552

Chromosomal Location

12p13.31

Expression System

Sf9

Tag

C-DDK

Related Pathways

Complement and coagulation cascades, ECM-receptor interaction, Focal adhesion

Field of Research

Complement and coagulation cascades, ECM-receptor interaction, Focal adhesion

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

50mM Tris-HCl, pH8.0, 100mM glycine, 10% glycerol

Function

Druggable Genome, Secreted Protein

Molecular Weight

67 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

VWF

Species

Human

Protein ID

NP_000543

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/7450

Uniprot URL

https://www.uniprot.org/uniprot/P04275

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_000552

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_000543

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