RIP3 (RIPK3) (NM_006871) Human Recombinant Protein

Product Specifications

Background

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of mutant (K50A) of Human receptor-interacting serine-threonine kinase 3 (RIPK3, K50A mutant), full length, with N-terminal GST and C-terminal His tag, expressed in sf9, 20ug

Synonyms

RIP3

Gene ID

11035

UniProt

Q9Y572

Accession Number mRNA

NM_006871

Chromosomal Location

14q12

Expression System

Sf9

Tag

N-GST

Related Pathways

Cytosolic DNA-sensing pathway

Field of Research

Cytosolic DNA-sensing pathway

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

50mM Tris-HCl, pH7.4, 20mM Imidazole, 0.3mM DTT, 10% glycerol

Function

Druggable Genome, Protein Kinase

Molecular Weight

56.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.