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LIF (NM_002309) Human Recombinant Protein

Selected Variant

PH32511E0

Size: 20 µg

Product Specifications

Background

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Overview

Recombinant protein of human leukemia inhibitory factor (LIF) produced in E. coli.

Synonyms

CDF; DIA; HILDA; MLPLI

Gene ID

3976

UniProt

P15018

Accession Number mRNA

NM_002309

Chromosomal Location

22q12.2

Expression System

E. coli

Tag

Tag Free

Related Pathways

Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

Field of Research

Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

Concentration

Resuspend the protein to the desired concentration in proper buffer.

Endotoxin

< 0.1 EU per 1 µg of the protein by the LAL

Purity

>95% as determined by SDS-PAGE and Coomassie blue staining

Activity

Determined by its ability to induce differentiation of M1 cells. ED50 for this effect is typically < 0.04 ng/ml.

Form

Lyophilized

Buffer

Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.

Function

Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein

Molecular Weight

19.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

LIF

Species

Human

Protein ID

NP_002300

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/3976

Uniprot URL

https://www.uniprot.org/uniprot/P15018

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_002309

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_002300

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