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UBE2E3 (NM_006357) Human Recombinant Protein

Product Specifications

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013]

Synonyms

UBCH9; UbcM2

Gene ID

10477

UniProt

Q969T4

Accession Number mRNA

NM_006357

Chromosomal Location

2q31.3

Expression System

E. coli

Tag

N-His

Related Pathways

Ubiquitin mediated proteolysis

Field of Research

Ubiquitin mediated proteolysis

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol

Molecular Weight

22.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

UBE2E3

Species

Human

Protein ID

NP_006348

Overview

Recombinant protein of human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/10477

Uniprot URL

https://www.uniprot.org/uniprot/Q969T4

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_006357

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_006348

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