MRPS14 (NM_022100) Human Recombinant Protein
Product Specifications
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
Synonyms
COXPD38; DJ262D12.2; HSMRPS14; MRP-S14; S14mt
Gene ID
63931
UniProt
O60783
Accession Number mRNA
NM_022100
Chromosomal Location
1q25.1
Expression System
E. coli
Tag
N-His
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl
Molecular Weight
15.1 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
MRPS14
Species
Human
Protein ID
NP_071383
Overview
Recombinant protein of human mitochondrial ribosomal protein S14 (MRPS14), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/63931
Uniprot URL
https://www.uniprot.org/uniprot/O60783
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_022100
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_071383
Curated Selection
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