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MRPS14 (NM_022100) Human Recombinant Protein

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Synonyms

COXPD38; DJ262D12.2; HSMRPS14; MRP-S14; S14mt

Gene ID

63931

UniProt

O60783

Accession Number mRNA

NM_022100

Chromosomal Location

1q25.1

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

15.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

MRPS14

Species

Human

Protein ID

NP_071383

Overview

Recombinant protein of human mitochondrial ribosomal protein S14 (MRPS14), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/63931

Uniprot URL

https://www.uniprot.org/uniprot/O60783

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_022100

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_071383

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