TRMT12 (NM_017956) Human Recombinant Protein

Product Specifications

Background

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006) .[supplied by OMIM, Mar 2008]

Overview

Recombinant protein of human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

TRM12; TYW2

Gene ID

55039

UniProt

Q53H54

Accession Number mRNA

NM_017956

Chromosomal Location

8q24.13

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

50.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.