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ATP6V1C2 (NM_144583) Human Recombinant Protein

Product Specifications

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. [provided by RefSeq, Jul 2008]

Synonyms

ATP6C2; VMA5

Gene ID

245973

UniProt

Q8NEY4

Accession Number mRNA

NM_144583

Chromosomal Location

2p25.1

Expression System

E. coli

Tag

N-His

Related Pathways

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Field of Research

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

48.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

ATP6V1C2

Species

Human

Protein ID

NP_653184

Overview

Recombinant protein of human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/245973

Uniprot URL

https://www.uniprot.org/uniprot/Q8NEY4

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_144583

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_653184

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