CDC6 (NM_001254) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human cell division cycle 6 homolog (S. cerevisiae) (CDC6), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

CDC18L; HsCDC6; HsCDC18; MGORS5

Gene ID

990

UniProt

Q99741, A0A024R1S2

Accession Number mRNA

NM_001254

Chromosomal Location

17q21.2

Expression System

E. coli

Tag

N-His

Related Pathways

Cell cycle

Field of Research

Cell cycle

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

62.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.