PRPK (TP53RK) (NM_033550) Human Recombinant Protein

Product Specifications

Background

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t (6) A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914) . The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914) . TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity) . Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806) .[UniProtKB/Swiss-Prot Function]

Overview

Recombinant protein of human TP53 regulating kinase (TP53RK), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK; TPRKB

Gene ID

112858

UniProt

Q96S44

Accession Number mRNA

NM_033550

Chromosomal Location

20q13.12

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Function

Druggable Genome, Protein Kinase

Molecular Weight

28 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.