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GLS2 (NM_013267) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Synonyms

GA; GLS; hLGA; LGA

Gene ID

27165

UniProt

Q9UI32

Accession Number mRNA

NM_013267

Chromosomal Location

12q13.3

Expression System

E. coli

Tag

N-His

Related Pathways

Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism

Field of Research

Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

66.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

GLS2

Species

Human

Protein ID

NP_037399

Overview

Recombinant protein of human glutaminase 2 (liver, mitochondrial) (GLS2), nuclear gene encoding mitochondrial protein, with N-terminal HIS tag, expressed in E.Coli, 50ug

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/27165

Uniprot URL

https://www.uniprot.org/uniprot/Q9UI32

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_013267

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_037399

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