SPI1 (NM_003120) Human Recombinant Protein

Product Specifications

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Overview

Recombinant protein of human spleen focus forming virus (SFFV) proviral integration oncogene spi1 (SPI1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

OF; PU.1; SFPI1; SPI-1; SPI-A

Gene ID

6688

UniProt

P17947

Accession Number mRNA

NM_003120

Chromosomal Location

11p11.2

Expression System

E. coli

Tag

N-His

Related Pathways

Acute myeloid leukemia, Pathways in cancer

Field of Research

Acute myeloid leukemia, Pathways in cancer

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Function

Transcription Factors

Molecular Weight

30.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.