RIOK2 (NM_018343) Human Recombinant Protein

Product Specifications

Background

Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402) . Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710) .[UniProtKB/Swiss-Prot Function]

Overview

Recombinant protein of human RIO kinase 2 (RIOK2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

RIO2

Gene ID

55781

UniProt

Q9BVS4

Accession Number mRNA

NM_018343

Chromosomal Location

5q15

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

Function

Druggable Genome, Protein Kinase

Molecular Weight

63.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.