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ALF (GTF2A1L) (NM_006872) Human Recombinant Protein

Product Specifications

Background

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Mar 2014]

Overview

Recombinant protein of human general transcription factor IIA, 1-like (GTF2A1L), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

ALF

Gene ID

11036

UniProt

Q9UNN4, A0A140VKA3

Accession Number mRNA

NM_006872

Chromosomal Location

2p16.3

Expression System

E. coli

Tag

N-His

Related Pathways

Basal transcription factors

Field of Research

Basal transcription factors

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

Function

Transcription Factors

Molecular Weight

52.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

GTF2A1L

Species

Human

Protein ID

NP_006863

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/11036

Uniprot URL

https://www.uniprot.org/uniprot/Q9UNN4

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_006872

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_006863

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