ALAS1 (NM_199166) Human Recombinant Protein

Product Specifications

Background

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

Overview

Purified recombinant protein of Homo sapiens aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

ALAS; ALAS-H; ALAS3; ALASH; MIG4

Gene ID

211

UniProt

P13196, Q5JAM2

Accession Number mRNA

NM_199166

Chromosomal Location

3p21.2

Expression System

E. coli

Tag

N-His

Related Pathways

Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism

Field of Research

Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

70.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.