ALAS1 (NM_199166) Human Recombinant Protein
Product Specifications
Background
This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
Synonyms
ALAS; ALAS-H; ALAS3; ALASH; MIG4
Gene ID
211
UniProt
P13196, Q5JAM2
Accession Number mRNA
NM_199166
Chromosomal Location
3p21.2
Expression System
E. coli
Tag
N-His
Related Pathways
Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism
Field of Research
Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl
Molecular Weight
70.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
ALAS1
Species
Human
Protein ID
NP_954635
Overview
Purified recombinant protein of Homo sapiens aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/211
Uniprot URL
https://www.uniprot.org/uniprot/P13196
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_199166
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_954635
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