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MRPL4 (NM_015956) Human Recombinant Protein

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

CGI-28; L4mt

Gene ID

51073

UniProt

Q9BYD3, A0A024R7C5

Accession Number mRNA

NM_015956

Chromosomal Location

19p13.2

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

Molecular Weight

34.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

MRPL4

Species

Human

Protein ID

NP_057040

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/51073

Uniprot URL

https://www.uniprot.org/uniprot/Q9BYD3

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_015956

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_057040

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