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PFKFB2 (NM_006212) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of Human 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

PFK-2/FBPase-2

Gene ID

5208

UniProt

O60825

Accession Number mRNA

NM_006212

Chromosomal Location

1q32.1

Expression System

E. coli

Tag

N-His

Related Pathways

Fructose and mannose metabolism

Field of Research

Fructose and mannose metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

Function

Druggable Genome

Molecular Weight

58.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

PFKFB2

Species

Human

Protein ID

NP_006203

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/5208

Uniprot URL

https://www.uniprot.org/uniprot/O60825

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_006212

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_006203

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