P4HA3 (NM_182904) Human Recombinant Protein
Product Specifications
Background
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Overview
Purified recombinant protein of Human prolyl 4-hydroxylase, alpha polypeptide III (P4HA3), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
Synonyms
C-P4H alpha III; collagen prolyl 4-hydroxylase alpha (III) ; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III; prolyl 4-hydroxylase, alpha III subunit; prolyl 4-hydroxylase, alpha polypeptide III
Gene ID
283208
UniProt
Q7Z4N8
Accession Number mRNA
NM_182904
Chromosomal Location
11q13.4
Expression System
E. coli
Tag
N-His
Related Pathways
Arginine and proline metabolism, Metabolic pathways
Field of Research
Arginine and proline metabolism, Metabolic pathways
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.
Function
Druggable Genome
Molecular Weight
59.3 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
P4HA3
Species
Human
Protein ID
NP_878907
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/283208
Uniprot URL
https://www.uniprot.org/uniprot/Q7Z4N8
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_182904
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_878907
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