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P4HA3 (NM_182904) Human Recombinant Protein

Product Specifications

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Overview

Purified recombinant protein of Human prolyl 4-hydroxylase, alpha polypeptide III (P4HA3), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

C-P4H alpha III; collagen prolyl 4-hydroxylase alpha (III) ; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III; prolyl 4-hydroxylase, alpha III subunit; prolyl 4-hydroxylase, alpha polypeptide III

Gene ID

283208

UniProt

Q7Z4N8

Accession Number mRNA

NM_182904

Chromosomal Location

11q13.4

Expression System

E. coli

Tag

N-His

Related Pathways

Arginine and proline metabolism, Metabolic pathways

Field of Research

Arginine and proline metabolism, Metabolic pathways

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

Function

Druggable Genome

Molecular Weight

59.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

P4HA3

Species

Human

Protein ID

NP_878907

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/283208

Uniprot URL

https://www.uniprot.org/uniprot/Q7Z4N8

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_182904

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_878907

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