PFKFB1 (NM_002625) Human Recombinant Protein

Product Specifications

Background

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]

Overview

Purified recombinant protein of Human 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 1 (PFKFB1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Synonyms

F6PK; HL2K; PFRX

Gene ID

5207

UniProt

P16118

Accession Number mRNA

NM_002625

Chromosomal Location

Xp11.21

Expression System

E. coli

Tag

N-His

Related Pathways

Fructose and mannose metabolism

Field of Research

Fructose and mannose metabolism

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

Function

Druggable Genome

Molecular Weight

54.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.