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NUF2 (NM_145697) Human Recombinant Protein

Product Specifications

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug

Synonyms

CDCA1; CT106; NUF2R

Gene ID

83540

UniProt

Q9BZD4

Accession Number mRNA

NM_145697

Chromosomal Location

1q23.3

Expression System

E. coli

Tag

N-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1 % Sarkosyl.

Function

Druggable Genome

Molecular Weight

54.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

NUF2

Species

Human

Protein ID

NP_663735

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/83540

Uniprot URL

https://www.uniprot.org/uniprot/Q9BZD4

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_145697

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_663735

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