ACOT1 (NM_001037161) Human Recombinant Protein

Product Specifications

Background

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Human acyl-CoA thioesterase 1 (ACOT1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug

Synonyms

ACH2; CTE-1; LACH2

Gene ID

641371

UniProt

Q86TX2, E9KL42

Accession Number mRNA

NM_001037161

Chromosomal Location

14q24.3

Expression System

E. coli

Tag

N-His

Related Pathways

Biosynthesis of unsaturated fatty acids

Field of Research

Biosynthesis of unsaturated fatty acids

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1 % Sarkosyl.

Molecular Weight

46.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.