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MRPS35 (NM_021821) Human Recombinant Protein

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010]

Overview

Purified recombinant protein of Human mitochondrial ribosomal protein S35 (MRPS35), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

Synonyms

HDCMD11P; MDS023; MRP-S28; MRPS28

Gene ID

60488

UniProt

P82673

Accession Number mRNA

NM_021821

Chromosomal Location

12p11.22

Expression System

E. coli

Tag

N-GST and C-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

Molecular Weight

62.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

MRPS35

Species

Human

Protein ID

NP_068593

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/60488

Uniprot URL

https://www.uniprot.org/uniprot/P82673

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_021821

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_068593

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