POLR3F (NM_006466) Human Recombinant Protein
Product Specifications
Background
The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Synonyms
C34; RPC6; RPC39
Gene ID
10621
UniProt
Q9H1D9
Accession Number mRNA
NM_006466
Chromosomal Location
20p11.23
Expression System
E. coli
Tag
N-GST and C-His
Related Pathways
Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Field of Research
Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.
Function
Transcription Factors
Molecular Weight
61.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
POLR3F
Species
Human
Protein ID
NP_006457
Overview
Purified recombinant protein of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/10621
Uniprot URL
https://www.uniprot.org/uniprot/Q9H1D9
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_006466
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_006457
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