POLR3F (NM_006466) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Overview

Purified recombinant protein of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

Synonyms

C34; RPC6; RPC39

Gene ID

10621

UniProt

Q9H1D9

Accession Number mRNA

NM_006466

Chromosomal Location

20p11.23

Expression System

E. coli

Tag

N-GST and C-His

Related Pathways

Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

Field of Research

Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

Function

Transcription Factors

Molecular Weight

61.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.