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ATP5MF (NM_004889) Human Recombinant Protein

Product Specifications

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene. [provided by RefSeq, Nov 2010]

Overview

Purified recombinant protein of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Synonyms

ATP5J2; ATP5JL

Gene ID

9551

UniProt

P56134

Accession Number mRNA

NM_004889

Chromosomal Location

7q22.1

Expression System

E. coli

Tag

N-GST and C-His

Related Pathways

Metabolic pathways, Oxidative phosphorylation

Field of Research

Metabolic pathways, Oxidative phosphorylation

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

Function

Transmembrane

Molecular Weight

38.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

ATP5MF

Species

Human

Protein ID

NP_004880

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/9551

Uniprot URL

https://www.uniprot.org/uniprot/P56134

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_004889

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_004880

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